![]() ![]() It is shown that the implementation of the CIP strategy enabled the re-use of the Capto Core 700 resin for up to ten cycles without any negative impact on the purified product. Here, its impact was assessed across the entire purification process which also included an ultrafiltration/diafiltration step. The success of the CIP strategy was ultimately verified at the laboratory scale. The best performing conditions, comprised of 30% n-propanol and ≥0.75 N NaOH, were deployed in cycling experiments, completed with miniature chromatography columns, to demonstrate their effectiveness. Here, we describe the deployment of high throughput (HT) techniques to screen CIP conditions for cleaning Capto? Core 700 resin exposed to clarified cell culture harvest of a SARS-CoV-2 vaccine candidate produced in Vero adherent cell culture. During the development of a SARS-CoV-2 vaccine candidate, at the height of the COVID-19 pandemic, raw materials shortages, including chromatography resins, necessitated the determination of a cleaning in place (CIP) strategy for a multimodal core-shell resin both rapidly and efficiently. Various diagnostic tests are available for the detection of coronaviruses infection which is mainly based on antigen detection or specific antibody detection. Animal CoVs have been known since the late 1930s. Transmission through the ocular surface is also possible. Most of the coronaviruses are transmitted through respiratory droplets during coughing or sneezing. During the twenty-first century, the world has witnessed two pandemics of coronaviruses (SARSCoV and SARS-CoV-2) and fatal sporadic outbreaks due to the emerging Middle East respiratory syndrome-CoV. Bats are well adapted anatomically and physiologically to CoVs. The CoVs have frequently crossed species barriers and some have emerged as important human pathogens. These viruses can infect the respiratory, gastrointestinal, hepatic and central nervous system of humans, livestock, birds, bats and other wild animals. Summary Coronaviruses (CoVs) are important pathogens for humans and vertebrate animals. ![]()
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